Reproduction is a fundamental process necessary for the creation of future generations. There is currently a knowledge gap in the molecular mechanisms of the early steps of fertilization. In the United States, more individuals are seeking Assisted Reproductive Technologies (ART), its use has more than doubled from 2012 to 2021 and the number of infants born who were conceived through ART has increased by 50% to 2% of all births annually in the U.S. The average cost of each In Vitro Fertilization (IVF) transfer is $10,000 and the success of a live-birth after the first transfer is just 41% and decreases with maternal age. Concerningly, male infertility is rising, the sperm count has dropped by 50% from 1973 to 20181. Fertility rates are dropping worldwide due to lifestyle changes, an increasing age in which people decide to become parents, and environmental factors. We need a stronger foundation of the early stages of fertilization in order to address this global challenge.  The molecular basis of mammalian fertilization is a large unsolved question in human biology. Fundamental knowledge of this event would advance outcomes in fertility treatments.